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Physics > Biological Physics

arXiv:1604.04878 (physics)
[Submitted on 17 Apr 2016]

Title:High-extinction VIPA-based Brillouin spectroscopy of turbid biological media

Authors:Antonio Fiore, Jitao Zhang, Peng Shao, Seok Hyun Yun, Giuliano Scarcelli
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Abstract:Brillouin microscopy has recently emerged as powerful technique to characterize the mechanical properties of biological tissue, cell and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouin spectrometers do not have sufficient spectral extinction to reject the predominant non-Brillouin scattered light of turbid media. To overcome this issue, we developed a spectrometer composed of a two VIPA stages and a multi-pass Fabry-Perot interferometer. The Fabry-Perot etalon acts as an ultra-narrow band-pass filter for Brillouin light with high spectral extinction and low loss. We report background-free Brillouin spectra from Intralipid solutions and up to 100 microns deep within chicken muscle tissue.
Subjects: Biological Physics (physics.bio-ph); Optics (physics.optics); Quantitative Methods (q-bio.QM)
Cite as: arXiv:1604.04878 [physics.bio-ph]
  (or arXiv:1604.04878v1 [physics.bio-ph] for this version)
  https://doi.org/10.48550/arXiv.1604.04878
Related DOI: https://doi.org/10.1063/1.4948353

Submission history

From: Antonio Fiore [view email]
[v1] Sun, 17 Apr 2016 14:59:58 UTC (509 KB)
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